Members of the germin-like protein family in brassica napus pdf

Identification and analysis of the germinlike gene family in soybean. Harmon2,6, wei sha1,3 and sixue chen2,4,6 1 college of life. The colocalisation of the blackleg resistance genes lepr3 and rlm2 in the b. Ijms free fulltext transcriptomewide identification of. A brassica mirna regulates plant growth and immunity. Brassica napus, bor transporter, b deficiency, transcriptional profile, b efficiency, gene function background boron b is an essential micronutrient for higher plants 1. Enhancing resistance to sclerotinia minor in peanut by.

A role of some family members in defense against pathogen attack had been proposed based on gene regulation studies and transgenic approaches. Cloning and expression analysis of zmglp1, a new germinlike. Germinlike proteins glps are watersoluble plant glycoproteins belonging. Major haplotype divergence including multiple germinlike protein genes, at the wheat sr2 adult plant stem rust resistance locus. Research article open access genomewide identification of.

Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum. Genomic organization and structural diversity of germinlike. Verticillium longisporum, the invisible threat to oilseed. Functional characterization of germin and germinlike protein. Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum steffen rietz, friederike e. Department of molecular phytopathology, institute of phytopathology, christianalbrechtsuniversitat of kiel, germany, hermann, rodewald str. The mapkkks comprise the largest family in the mapk cascades, yet only a few of these genes have been associated with physiological functions, even in the model plant arabidopsis thaliana. Ijms free fulltext genomewide characterization and. Segmental structure of the brassica napus genome based on. Pei y, li x, zhu y, ge x, sun y, liu n, jia y, li f and hou y 2019 ghabp19, a novel germinlike protein from gossypium hirsutum, plays an important role in the regulation of resistance to verticillium and fusarium wilt pathogens. An increased production of plant secondary metabolites in response to v.

Sclerotinia stem rot ssr caused by the phytopathogenic fungus sclerotinia sclerotiorum is a major disease of oilseed rape brassica napus. This study describes the identification of a family of 14 germinlike genes from brassica napus bnglp designated bnglp1bnglp14 and investigated potential functions of bnglps in plant defense. The bhlh gene family has been identified in many species, except for brassica oleracea and b. The multigene family encoding germinlike proteins of. Read sunflower germinlike protein haglp1 promotes ros accumulation and enhances protection against fungal pathogens in transgenic arabidopsis thaliana, plant cell reports on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.

Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia. Previous studies have shown that germinlike proteins glps are present ubiquitously in rice and arabidopsis. A total of 21,237 sage tags were sequenced and 4,211 unique tags were identified. The index is used to find areas of probable homology, which are then loaded into memory for a detailed alignment. Identification and characterization of micrornas in oilseed. In this study, proteins from xylem sap of adult brassica napus plants were precipitated by acetone, separated by 2de and partial amino acid sequences were determined by tandem mass spectrometry. Bootstrap confidence values were obtained using replicates and only more than 75% of. Pdf members of the germinlike protein family in brassica. The important role of glps in plant responses against various abiotic and biotic stresses, especially pathogens, is well validated. In australia, other important cropping weeds from the brassicaceae family include hirschfeldia incana, diplotaxis spp. Taro is a tropical root crop that is grown widely throughout the pacific, africa, asia, the west indies, and south america plucknett et al. They are part of the cupin superfamily of biochemically diverse proteins, a superfamily that has a conserved tertiary structure, though with limited similarity in primary sequence. Chromosome geographical name count distribution habitat b. A protein blast search of rsppd against the arabidopsis proteome uncovered high homology to members of the mes family of arabidopsis vlot et al.

We chose to work with the burp family for a variety of reasons. Functional characterization of germin and germinlike. Use of serial analysis of gene expression technology to. Identification, structure, and differential expression of. The aim of our study was to investigate extracellular defence reactions induced by v. Pdf functional characterization of germin and germinlike protein.

Genomewide characterization and expression analysis of the. The brad gff3 varies so widely in its attribute formatting from most gff3 files that ive hardly been able to get it to work with rnaseq related software. Rietz s, bernsdorff fe, cai d 2012 members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum. The basic helixloophelix bhlh is the second largest gene family in the plant, some members play important roles in pistil development and response to drought, waterlogging, cold stress and salt stress. Ghabp19, a novel germinlike protein from gossypium. Taro colocasia esculenta transformed with a wheat oxalate. Brassica napus glp genes are induced as early as 6 hpi with s.

Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of. Phylogenetic tree of members of the mir169 family in brassica napus and arabidopsis thaliana based on their precursor sequences using the neighbor. Significant sequence similarity is observed with enoyl acyl carrier protein reductase enr, the other reductase of fas, and the shortchain alcohol dehydrogenase sdr family. Proteomic approach to understand the molecular physiology. Comparative analysis of cdresponsive maize and rice.

Factors affecting pathogenicity of the turfgrass dollar spot. In the present study, we report genomewide identification, characterisation, subcellular localization, enzyme activity, and expression analysis of the glp gene family. Rietz s, bernsdorff fem, cai d 2012 members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum. Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis. Identification and characterization of micrornas in. Using the yeast two hybrid system, we isolated two members of the thioredoxinh family that interact specifically with the srk910 protein kinase domain. Bernsdorff and daguang cai, members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum, journal of experimental botany, 63, 15, 5507, 2012. Background germinlike superfamily members are ubiquitously. As demonstrated before, the xylem sap collected with the employed method showed no detectable contaminations from phloem sap or other. Examples of the diversity and characteristics of selected natural brassica species. Jul 31, 2014 phylogenetic tree of members of the mir169 family in brassica napus and arabidopsis thaliana based on their precursor sequences using the neighbor.

Brassica as a crop and raphanus, sinapis, and brassica as weeds. However, little is known about cotton glps in relation to fungal pathogens. Mar 31, 2016 members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum. Integrated mrna, srna, and degradome sequencing reveal. The brassica napus receptorlike protein rlm2 is encoded by a second. Worldwide, canola is the third most important edible. First, identification and characterization of additional members of the burp gene family in soybean could be of practical importance. Analysis of xylem sap proteins from brassica napus bmc. Here, a novel glp gene was isolated from gossypium hirsutum and designated as ghabp19. Transient expression of members of the germinlike gene family in epidermal cells of wheat confers disease resistance. Pdf the important role of germins ger and genes coding for germinlike proteins glp in responses against. In the present study, twentyone germin gene members in soybean cultivar.

Three droughtresponsive members of the nonspecific lipid. Like most of jims software interactive use on this web server is. Caimembers of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum. Members of the germinlike protein family in brassica napus are candidates. Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum regulation of two germinlike protein genes during plum fruit development regulation of two germinlike protein genes during plum fruit development. Sclerotinia minor jagger is the causal agent of sclerotinia blight, a highly destructive disease of peanut arachis hypogaea. Because of extensive sequence information from genome or expressed sequence tag est sequencing programs, it became clear that germin and glps belong to a multigene family in plants, including arabidopsis arabidopsis thaliana, rice oryza sativa, and barley subsp.

During infection, large, whitegrey lesions form on the stems of the host plant, perturbing seed development and decreasing yield. The control of sclerotinia stem rot on oilseed rape brassica. Jun 30, 2016 read tmtbased quantitative proteomics analyses reveal novel defense mechanisms of brassica napus against the devastating necrotrophic pathogen sclerotinia sclerotiorum, journal of proteomics on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Microbial relatives of the seed storage proteins of higher.

Brassica napus, germinlike proteins glps, oxidative burst, plant disease resistance, sclerotinia sclerotiorum. This work demonstrates that a brassica mirna, which is subject to dynamic regulation during development and responds to viral infection, regulates immunity and development through distinct modes of action. Members of the germinlike protein family in brassica napus are. There are also horticultural cultivars used as vegetable crops including turnip and rapini or broccoli raab. Germin and germinlike proteins constitute a ubiquitous family of plant proteins. Xylem protein extraction, separation and identification. The genus name comes from the classical latin word brassica, referring to cabbage and other cruciferous vegetables. Interestingly, the number of unique tags in pollen was low compared with the sage library of the leaf constructed on a similar scale. Two members of the thioredoxinh family lnteract with the. Based on evidence that oxalic acid is involved in the pathogenicity of many sclerotinia species, our objectives were to recover transgenic peanut plants expressing an oxalic aciddegrading oxalate oxidase and to evaluate them for increased resistance to s.

Nectarin i is a novel, soluble germinlike protein expressed in the nectar of nicotiana sp. Overexpression of germinlike protein gmglp10 enhances. Thereby, rsppd clustered with the members of subfamily 2, as determined by yang et al. Germinlike proteins glps are ubiquitous watersoluble glycoproteins that are located in the extracellular matrix. Members of the germinlike protein family in brassica napus. Brassica napus is one of the most importantly economical and biofuel crops. Mes16, a member of the methylesterase protein family. To identify bnglp members with a putative function in defence against s. This study aims to identify the bhlh family members in b. Identification and functional analysis of mitogenactivated. Free fulltext pdf articles from hundreds of disciplines, all in one place identification and functional analysis of mitogenactivated protein kinase kinase kinase mapkkk genes in canola brassica napus l. Characterization of peanut germinlike proteins, ahglps in. Identification and analysis of mkk and mpk gene families in.

Bootstrap confidence values were obtained using replicates and only more than 75% of replications are shown. It was the fifth most harvested root crop in the world with production estimated at 9. Molecular cloning and characterization of novel morus alba. Germin and germinlike proteins glps are encoded by a family of genes found in all plants. The brassica genus consists of approximately 100 species, including species brassica napus l. Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum pp. Graham king southern cross university provides no express or implied warranties of any kind, including but not limited to those of merchantability, fitness for a particular purpose and noninfringement of the proprietary rights, such as s, patents and trade secrets, of third parties. Ive generated genome indices via star using two brassica napus reference genome sources. Genomewide identification of brassica napus micrornas and. Identification and analysis of the germinlike gene family. Transcriptomic comparison between brassica oleracea and rice.

Genomewide identification and characterization of nodule. Origins and diversity of brassica and its relatives 5 table 1. A role of some family members in defense against pathogen attack. Regulation of two germinlike protein genes during plum fruit development regulation of two germinlike protein genes during plum fruit development. Jun, 2015 rietz s, bernsdorff fem, cai d 2012 members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum. Identification and analysis of the germinlike gene family in. The tradeoff between growth and defense has long been a major challenge in crop breeding. Comparative analysis of basic helixloophelix gene family. Dec 18, 2008 verticillium longisporum is one of the most important pathogens of brassicaceae that remains strictly in the xylem during most stages of its development. The protein index takes a little more than 2 gigabytes. However, the understanding regarding their role in development and abioticbiotic stress resistance remains limited.

The fungal pathogen sclerotinia sclerotiorum infects a broad range of dicotyledonous plant species and causes stem rot in brassica napus. Characterization of peanut germinlike proteins, ahglps in plant. The brassica napus bkr enzyme is nadphdependent and forms part of a dissociable type ii fatty acid synthetase fas. The brassica genus is a member of brassicaceae cruciferae and contains 39 species. Protein blat works in a similar manner, except with 4mers rather than 11mers. Members of the germinlike protein family in brassica. Pdf germin and germinlike proteins constitute a ubiquitous family of plant proteins. Relationship between disease resistance and rice oxalate. In this study, we identified a total of 34 fulllength aap genes representing eight subgroups aap18 from the allotetraploid rapeseed genome a na nc nc n,2n4x38. Pdf characterization of peanut germinlike proteins. Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum article fulltext available. This document provides an overview of baseline biological information relevant to risk assessment of genetically modified forms of the species that may be released into the australian environment. In the present study, twentyone germin gene members in.

Pdf identification and analysis of the germinlike gene family in. To explore mapkkk functions in biotic and abiotic stress. May 26, 2016 rietz s, bernsdorff fe, cai d 2012 members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum. The name germin was given because the first described member of the family, wheat germin, was discovered in germinating wheat grains. In the present study, a glp in soybean glycine max l. In the available sequence databases, we identified a germinlike gene family in b.

Germins and germinlike proteins glps constitute a large and highly diverse family of ubiquitous plant proteins. Comprehensive analyses of the bes1 gene family in brassica. Brassica napus, germinlike proteins glps, oxidative burst, plant disease resistance, sclerotinia sclerotiorum, superoxide dismutase. Frontiers ghabp19, a novel germinlike protein from. However, few systematic analyses of aaps have been reported in brassica napus so far. We have characterized the global gene expression patterns of arabidopsis pollen using serial analysis of gene expression sage.

In the present study, twentyone germin gene members in soybean. Frontiers emerging trends in molecular interactions between. Research article open access genomewide identification. Brassica napus xylem sap contains only low concentrations of amino acids, and aromatic amino acids are especially scarce singh et al. Colocalisation of the blackleg resistance genes rlm2 and. In the present study, the characterization of the spatial, developmental, drought, and abainduced expression of three nsltp gene family members are presented.

Germin like proteins glps are watersoluble plant glycoproteins belonging to the cupin superfamily. It has been suggested that disease symptoms are associated with clogging of xylem vessels. These proteins have been reported to play vital roles in diverse biological processes. The adult plant stem rust resistance gene sr2 was introgressed into hexaploid wheat cultivar cv marquis from tetraploid emmer wheat cv yaroslav, to generate stem rust resistant cv hope in the 1920s. The low affinity sulfate transporter last 3 family protein encoded by genes in a gene cluster was identified as the target of two distinct mirnas ptomir395a and ptomir395bk. Oct 01, 2015 read sunflower germinlike protein haglp1 promotes ros accumulation and enhances protection against fungal pathogens in transgenic arabidopsis thaliana, plant cell reports on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Segmental structure of the brassica napus genome based on comparative analysis with arabidopsis thaliana isobel a. Nucleotide sequence of a member of the napin storage protein family from brassica napus received for publication, december 19, 1986. The journal of biological chemistry 0 1967 by the american society for biochemistry and molecular biology, inc. Tmtbased quantitative proteomics analyses reveal novel. Expression of an oxalate oxidase gene in tomato and. Arabidopsis thaliana contains a large family of germinlike proteins. It is therefore possible that different gene family members account for the observed diversity in patterns of expression, each one perhaps performing a different function.